Overview of Analytical Methods for Characterizing the Charge Heterogeneity of Antibody-Drug Conjugates
Ayat Abbood
Ayat Abbood, Department of Medicinal Chemistry and Quality Control, Faculty of Pharmacy, University of Tishreen, Latakia, Syria.
Manuscript received on 05 July 2024 | Revised Manuscript received on 17 July 2024 | Manuscript Accepted on 15 August 2024 | Manuscript published on 30 August 2024 | PP: 16-22 | Volume-4 Issue-5, August 2024 | Retrieval Number: 100.1/ijapsr.E404704050824 | DOI: 10.54105/ijapsr.E4047.04050824
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Abstract: Antibody-drug conjugates (ADCs) are ideal candidates for selective therapy. ADCs display considerable heterogeneity resulting from various modifications. One of the molecular characteristics of ADCs is charge heterogeneity. Several analytical methods could be used to characterize the charge heterogeneity of ADC including ion exchange chromatography (IEC), isoelectric focusing (IEF), and capillary electrophoresis (CE). This work aimed to give insights into the different analytical techniques used to determine pI and the charge distribution profile of ADCs.
Keywords: Antibody, Conjugates, Charge, Heterogeneity.
Scope of the Article: Medicinal Chemistry