Analysis of Charge Variant Profiles in Antibody Conjugates
Ayat Abbood
Prof. Ayat Abbood, Department of Medicinal Chemistry and Quality Control, Faculty of Pharmacy, University of Tishreen, Latakia, Syria.
Manuscript received on 24 January 2025 | First Revised Manuscript received on 12 February 2025 | Second Revised Manuscript received on 19 March 2025 | Manuscript Accepted on 15 April 2025 | Manuscript published on 30 April 2025 | PP: 26-32 | Volume-5 Issue-3, April 2025 | Retrieval Number: 100.1/ijapsr.C407205030425 | DOI: 10.54105/ijapsr.C4072.05030425
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© The Authors. Published by Lattice Science Publication (LSP). This is an open-access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Abstract: The aim of this study was to analyze the charge variant profiles of both unconjugated and conjugated antibodies. The first antibody exhibited the lowest alkalinity, with isoelectric point (pI) values ranging from 7.7 to 8.2. This was followed by the second antibody, which had pI values between 8.3 and 8.6. The third antibody was identified as the most alkaline, displaying pI values from 8.95 to 9.00. The first antibody was conjugated to a maytansine derivative via linkers, while the second and third antibodies were linked to tomaymycin. The conjugated antibodies demonstrated increased heterogeneity and more acidic characteristics compared to their unconjugated counterparts. Specifically, the first antibody conjugated with the maytansine derivative had a pI range of 7.0 to 8.1 (∆pI: 1.1), the second antibody with tomaymycin ranged from 7.8 to 8.4 (∆pI: 0.6), and the third antibody had pI values from 8.3 to 8.8 (∆pI: 0.5). The charge variant profiles obtained through imaging capillary isoelectric focusing exhibited high repeatability both within and across days.
Keywords: Antibody, Conjugates, Charge Variants, pI.
Scope of the Article: Pharmaceutical Biotechnology